Anass Assaidi, Medical Doctor (PhD Medicine), is an Assistant Professor of Interventional Cardiology at Mohammed VI University of Health Sciences and Cheikh Khalifa ibn Zaid Hospital. He is a Graduate Cardiologist from Aix Marseille II University and Casablanca Medicine University, got Interuniversity Diploma of "Pediatric and Congenital Cardiology" in 2012 from Aix-Marseille II University, “Echocardiography" from Bordeaux II University and "Endovascular treatment of valvular heart disease" from Paris V University. He is an active member of European, French & Moroccan Societies of Cardiology and Member of the Interventional Cardiology Reflection Group. He participated in many research projects related to cardiovascular diseases and author of several publications.
Introduction: Several studies confirm that combining ivabradine with beta-blockers, reduce more effectively the heart rate in patients with heart failure and/or angina than beta-blockers alone. Methods: Non-interventional cohort study, conducted in Morocco cardiology clinical practice, in patients with heart failure or angina treated with ivabradine in combination with beta-blockers. The primary outcome, measured at 1 and 4 months, were HR, NYHA classification, and SEATTLE quality of life questionnaire for angina patients. Results: 497 patients were included, their mean age was 65.6±10.3 years, mean HR was 89.4±14.5 bpm and 61.6% were male. 10.9% treated with less than the initial beta-blockers dosage, 56.8% under initial dosage, 21.9% under less than recommended dosage and 10.4% under recommended dose. After 4 months, the mean HR showed a significant reduction of 27±13.8 bpm (p<0.0001) and no statistical significant difference (p=0.46) observed between beta-blockers dosages which was respectively 26.2±0.8, 28.2±2, 32.2±2, and 26.08±1.3, for initial, recommended, less than initial and less than recommended dosage. 42% of patients became NYHA I, 55% NYHA II, only 4% were NYHA III and no patient remained NYHA IV regardless of BB dosages. There was no-significant difference associated to the BB dose (p=0.53) in the 5 SAQ domain-scores between baseline (V0) and 4 months follow up visit (V2). Conclusion: In this study, ivabradine was effective in reducing HR in CHF and angina patients over a period of 4 months irrespective of the dose of beta-blockers. There was a marked shift from higher to lower NYHA classes independently of the BB administered dose.
Jana Mrzílková has completed her MD and Post-doctoral studies from Charles University, Prague, Czech republic. She is the Director of Experimental Micro CT laboratory at Third Faculty of Medicine, Charles University, Prague, Czech republic. She has published more than 8 papers in reputed journals and has been also involved in neuroscience research.
During past years, several staining methods were developed in order to increase the contrast of soft tissues. However, most of these methods are complicated, time-consuming and use toxic contrast agents. One of the best and mostly used contrasts agents for soft tissue imaging are aqueous solutions of osmium tetroxide, phosphomolybdic acid (PMA) or phosphotungstic acid (PTA). Osmium tetroxide is very toxic, does not stain well if samples have been in alcohol and also its penetration is slow. PTA penetrates tissues slowly also, but it is less toxic, simpler to use and effectively stains alcohol-stored samples. PMA gives better contrast among different tissues, but requires longer incubation; conversely, its contrast between different tissues was superior. Our goal was to create a simple, cheap and stable fixation method for ex-vivo soft tissues scanning in micro-CT, a method which would give sufficient contrast among soft tissues in organs. Ethanol provided contrast enhancement in both studied organs in all used types of fixation. Fixation in 97% ethanol enhanced contrast among the tissues already after 72 hours, however, it caused hardening of the organs and in some cases even rupture of the specimens. Fixation in 50% ethanol provided best results after 336 hours, and details were not visualized as well as in 97% ethanol; conversely, samples were not that stiff. Best results provided fixation in a row of ascending ethanol concentrations; all organs were visualized in great details without being damaged.